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Related Experiment Videos

Cell and tissue responses to electric shocks.

Takashi Ashihara1, Natalia A Trayanova

  • 1Department of Biomedical Engineering, Tulane University, Boggs Center, New Orleans, LA 70118, USA.

Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology
|August 17, 2005
PubMed
Summary

New models incorporating electroporation and an outward current (I(a)) accurately simulate myocardial membrane kinetics and shock-induced electrical activity. These advancements improve understanding of cardiac electrophysiology and reduce the threshold for shock-induced excitation.

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Area of Science:

  • Cardiac electrophysiology
  • Computational modeling
  • Membrane biophysics

Background:

  • Existing myocardial membrane models fail to replicate experimental findings of negative bias in transmembrane potential changes (DeltaV(m)) after strong electric shocks.
  • Understanding these discrepancies is crucial for accurate modeling of cardiac electrical behavior during defibrillation.

Purpose of the Study:

  • To integrate electroporation and an outward current (I(a)), part of K(+) flow through L-type Ca(2+)-channels, into existing membrane models.
  • To evaluate the impact of these additions on simulating shock-induced transmembrane potential changes and break excitation in a 2D cardiac preparation.

Main Methods:

  • Simulations were performed using the Luo-Rudy dynamic model (LRd'2000) for membrane dynamics.
  • Electroporation (LRd + EP model) and an outward current (aLRd model) were sequentially added to the baseline model.

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  • The models were tested in bidomain fiber and sheet simulations subjected to electrical shocks.

    • Main Results:

    • The modified aLRd model successfully reproduced the experimentally observed rectangularly-shaped positive DeltaV(m) and the negative-to-positive DeltaV(m) ratio.
    • The outward current (I(a)) contributed to the negative bias in DeltaV(m) asymmetry and restricted positive DeltaV(m).
    • Electroporation decreased the cathode-break excitation threshold and shifted excitation occurrence to post-shock in the aLRd model.

    Conclusions:

    • Incorporating electroporation and the outward current (I(a)) into membrane models aligns simulation results with experimental data.
    • The aLRd model provides a more accurate representation of myocardial membrane kinetics under strong electrical stimulation.
    • The enhanced model demonstrates a reduced threshold for shock-induced break excitation.