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Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging
Published on: June 20, 2016
How to measure Ca2+ in cellular organelles?
Oleg Gerasimenko1, Alexei Tepikin
1The Physiological Laboratory, The University of Liverpool, Crown Street, P.O. Box 147, Liverpool L69 3BX, UK.
Abstract:
The review will aim to briefly summarise information on calcium measurements in cellular organelles with emphases on studies conducted in live cells using optical probes. When appropriate we will try to compare the effectiveness of different indicators for intraorganellar calcium measurements. We will consider calcium measurements in endoplasmic reticulum, Golgi apparatus, endosomes/lysosomes, nucleoplasm, nuclear envelope, mitochondria and secretory granules.
