Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polymorphisms of human cholesterol 7 alpha-hydroxylase.

W G Karam1, J Y Chiang

  • 1Department of Biochemistry and Molecular Pathology, Northeastern Ohio Universities College of Medicine, Rootstown 44272.

Biochemical and Biophysical Research Communications
|June 15, 1992
PubMed
Summary

Researchers identified genetic variations in human liver cholesterol 7 alpha-hydroxylase (CYP7) cDNAs. These findings suggest potential allelic differences in the CYP7 gene, impacting cholesterol metabolism.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhaled corticosteroids and the increased risk of pulmonary tuberculosis: a population-based case-control study.

International journal of clinical practice·2014
Same author

Cytology and clinical spectrum of sexually transmitted infections in Lebanese women as revealed by Pap smear: a cross-sectional study from 2002-2006.

Journal of biological regulators and homeostatic agents·2011
Same author

Transcriptional regulation of the human sterol 12alpha-hydroxylase gene (CYP8B1): roles of heaptocyte nuclear factor 4alpha in mediating bile acid repression.

The Journal of biological chemistry·2001
Same author

Nuclear receptor-mediated repression of human cholesterol 7alpha-hydroxylase gene transcription by bile acids.

Journal of lipid research·2001
Same author

Transcriptional regulation of human oxysterol 7 alpha-hydroxylase gene (CYP7B1) by Sp1.

Gene·2001
Same author

Regulation of cholesterol 7alpha-hydroxylase gene (CYP7A1) transcription by the liver orphan receptor (LXRalpha).

Gene·2001

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Cholesterol 7 alpha-hydroxylase (CYP7) is a key enzyme in bile acid synthesis.
  • Understanding CYP7 gene variations is crucial for comprehending cholesterol homeostasis.

Purpose of the Study:

  • To isolate and characterize human liver cholesterol 7 alpha-hydroxylase (CYP7) cDNAs.
  • To identify and analyze sequence variations within the CYP7 gene.

Main Methods:

  • Isolation of full-length CYP7 cDNAs from a human liver cDNA library.
  • Nucleotide sequencing to determine cDNA sequences and identify variations.
  • Comparison of identified sequences with previously reported data.

Main Results:

Related Experiment Videos

  • A full-length CYP7 cDNA of 2901 nucleotides encoding a 504-amino acid P450 polypeptide was identified.
  • Two distinct sequences (Phe and Ser) were found at codon 100, suggesting allelic polymorphism.
  • Observed differences in codons 347 and 385 compared to previously published sequences.

    • Conclusions:

    • The identified variations at codon 100 likely represent polymorphisms arising from two different alleles of the human CYP7 gene.
    • These findings contribute to the understanding of genetic diversity in human CYP7 and its implications for cholesterol metabolism.