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A functional annotation of subproteomes in human plasma
Peipei Ping1, Thomas M Vondriska, Chad J Creighton
1Department of Physiology and Medicine, Division of Cardiology, Cardiovascular Research Laboratories, University of California, Los Angeles, CA, USA.
Proteomics
|August 17, 2005
Summary
This study provides a functional annotation of the human plasma proteome, identifying protein sources and interactions. The findings enhance the Plasma Proteome Database, aiding future research in proteomics and biomarker discovery.
Area of Science:
- Proteomics
- Bioinformatics
- Molecular Biology
Background:
- The Human Proteome Organization's Plasma Proteome Pilot project (HPPP) generated extensive data on plasma proteins.
- A comprehensive understanding of the human plasma proteome's function and interactions is crucial for biological and medical research.
Purpose of the Study:
- To perform a functional annotation of the human plasma proteome using HPPP data.
- To identify protein sources, develop a protein interaction network, and annotate proteins within key biological pathways.
Main Methods:
- Bioinformatic analyses for protein source determination and network construction.
- Functional subproteome annotation (coagulation, phagocytic system, inflammation, cardiovascular, liver, DNA binding).
- Application of multi-lectin chromatography and peptidomic techniques for protein enrichment and characterization.
Main Results:
- Development of a functional annotation of the human plasma proteome.
- Creation of a protein interaction network and identification of protein sources.
- Contribution of data to the Plasma Proteome Database, including analysis of post-translational modifications.
Conclusions:
- The study successfully annotated the human plasma proteome, revealing insights into protein functions and interactions.
- Methodological advancements in protein enrichment and characterization were addressed.
- The collaborative HPPP effort significantly advanced plasma proteome research.