Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression differences in mice divergently selected for methamphetamine sensitivity.

Abraham A Palmer1, Miguel Verbitsky, Rathi Suresh

  • 1Columbia Genome Center, Columbia University, 1150 St. Nicholas Ave., New York, New York 10032, USA. aap2010@columbia.edu

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|August 18, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Data-driven RNA phenotyping captures genetically regulated dimensions of the transcriptome.

American journal of human genetics·2026
Same author

Comparison of Deep Learning Tools for Optic Nerve Axon Quantification Finds Limited Generalizability Upon Independent Validation.

Bioengineering (Basel, Switzerland)·2026
Same author

Dissecting the Strain and Sex Specific Connectome Signatures of Unanesthetized C57BL/6J and DBA/2J Mice Using Magnetic Resonance Imaging.

Genes, brain, and behavior·2026
Same author

Erratum to Loss of GalNAc-T14 links O-glycosylation defects to alterations in B cell homing in IgA nephropathy.

The Journal of clinical investigation·2026
Same author

Genome-wide association study of cocaine self-administration behavior in Heterogeneous Stock rats.

Nature communications·2026
Same author

Genetic Architecture of Addiction-Relevant Behaviors in Outbred Sprague-Dawley Rats Reveals Loci for Anxiety-Like and Nociceptive Traits.

Genes, brain, and behavior·2026

Researchers identified genes linked to methamphetamine (MA) sensitivity by mapping behavioral quantitative trait loci (bQTL) and analyzing gene expression in mice. An expression QTL for Csnkle on Chromosome 15 co-mapped with a bQTL for MA stimulation, suggesting a shared genetic cause.

Area of Science:

  • Genetics
  • Neuroscience
  • Pharmacology

Background:

  • Drug abuse liability is a complex trait influenced by genetic factors.
  • Understanding the genetic basis of stimulant sensitivity is crucial for addiction research.

Purpose of the Study:

  • To identify genes associated with methamphetamine (MA) sensitivity and drug-abuse liability.
  • To map behavioral quantitative trait loci (bQTL) for MA-induced locomotor activity.
  • To investigate gene expression differences in the nucleus accumbens between mouse lines with high and low MA activity.

Main Methods:

  • Selective breeding of mice for high (HMACT) and low (LMACT) MA-induced activity.
  • Gene expression analysis using Affymetrix microarrays and quantitative polymerase chain reaction (qPCR).

Related Experiment Videos

  • Bioinformatic analysis using the WebQTL.org database to identify expression quantitative trait loci (eQTL).

    • Main Results:

    • Expression differences were observed for genes including Casein Kinase 1 Epsilon (Csnkle), Glutamate Receptor, Ionotropic, AMPA1 (GluR1), GABA B1 receptor (Gabbr1), and Dopamine- and cAMP-regulated phosphoprotein of 32 kDa (Darpp-32).
    • An eQTL for Csnkle on Chromosome 15 (LOD = 3.8) co-mapped with a bQTL for MA stimulation (LOD = 4.5).
    • This chromosomal region is also associated with cocaine sensitivity, suggesting a shared genetic basis for stimulant responses.

    Conclusions:

    • Altered gene expression, potentially due to gene expression polymorphisms, likely underlies the observed differences in MA sensitivity.
    • Combining genetic mapping, gene expression analysis, and bioinformatics is a powerful approach for identifying genes influencing complex traits.
    • The identified genes, particularly Csnkle, are strong candidates for further research into drug abuse liability and stimulant response.