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Updated: Aug 16, 2026
Increasing Pulmonary Artery Pulsatile Flow Improves Hypoxic Pulmonary Hypertension in Piglets
Published on: May 11, 2015
[Preliminary report: effects of artificial blood on hypoxic pulmonary vasoconstriction]
Yoshiaki Oshima1, Naoto Okazaki
1Department of Anesthesiology, Shimane Prefectural Central Hospital, Izumo 693-8555.
Background:
We examined the effects of artificial blood on hypoxic pulmonary vasoconstriction (HPV).
Methods:
Pump-perfused rabbit lungs were reperfused and ventilated with a mixture of 21% O2, 5% CO2, and balance N2. HPV was induced by reduction of O2 concentration from 21 to 3% for 5 min. Pulmonary arterial pressure (PAP) was measured using a low-pressure transducer. Two perfusion solutions, (1) a mixture of autologous red blood cells and physiological salt solution supplemented with 5% albumin and (2) artificial red blood cells (liposome-encapsulated hemoglobin) and physiological saline supplemented with 5% albumin, were made. Using each solution, HPV was evaluated by PAP increase with and without inhalation of nitric oxide (NO).
Results:
(1) With both autologous and artificial perfusion solutions, hypoxic stimulation (HS) increased PAP. (2) With the autologous solution, NO inhalation suppressed the HS-induced PAP increase, but with the artificial solutions no such a phenomenon was observed.
Conclusions:
NO inhalation failed to suppress HPV with the artificial solution, probably since the liposome-encapsulated hemoglobin strongly inactivated NO.
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