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A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis
Published on: March 24, 2017
Microplate nitrate reductase assay versus Alamar Blue assay for MIC determination of Mycobacterium tuberculosis
1Biotechnology Division, Regional Research Laboratory, Jammu Tawi, India.
Abstract:
A rapid colorimetric, microplate based nitrate reductase assay (NRA) method for minimum inhibitory concentration (MIC) determination of clinical isolates of Mycobacterium tuberculosis was compared with the microplate Alamar Blue assay (MABA), presently in vogue. Reproducible results were obtained in 7 days by NRA. The NRA method was found to be inexpensive, suitable for MIC determination against M. tuberculosis and can be suggested as an ideal substitute to MABA.
