Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Screening method for beta-lactamase substrate profiles.

H Schumacher1, M Nir, B Mansa

  • 1Department of Hospital Infections and Antibiotics, Immunological R & D, Copenhagen, Denmark.

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
|June 1, 1992
PubMed
Summary

A new disc diffusion method reliably screens beta-lactamase enzyme profiles for epidemiological studies. This technique differentiates various beta-lactamases, including TEM-1, SHV-1, and BRO-1, from clinical samples.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developing a web-based add-on intervention for parents of adolescents with anorexia nervosa: a mixed methods preliminary study.

Journal of eating disorders·2026
Same author

Low resting heart rates are associated with new-onset atrial fibrillation in patients with vascular disease: results of the ONTARGET/TRANSCEND studies.

Journal of internal medicine·2015
Same author

Resting heart rate is associated with renal disease outcomes in patients with vascular disease: results of the ONTARGET and TRANSCEND studies.

Journal of internal medicine·2014
Same author

Endovascular repair of acute uncomplicated aortic type B dissection promotes aortic remodelling: 1 year results of the ADSORB trial.

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2014
Same author

Early combination therapy with telmisartan plus amlodipine for rapid achievement of blood pressure goals.

International journal of clinical practice·2013
Same author

[Improve your skills!: evaluation of a 2.5-day basic course in vascular surgery for surgical trainees].

Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2013

Area of Science:

  • Microbiology
  • Enzymology
  • Epidemiology

Background:

  • Beta-lactamases are enzymes conferring antibiotic resistance.
  • Accurate identification of beta-lactamase types is crucial for epidemiological surveillance.
  • Existing methods may require complex procedures for substrate profiling.

Purpose of the Study:

  • To develop and validate a disc diffusion method for screening beta-lactamase substrate profiles.
  • To assess the method's utility in epidemiological studies and clinical isolate identification.

Main Methods:

  • A disc diffusion assay was adapted from Klundert's concept.
  • The method was tested using 30 reference beta-lactamases.
  • Validation included 59 clinical isolates producing TEM-1, SHV-1, and BRO-1 beta-lactamases.

Related Experiment Videos

Main Results:

  • The disc diffusion method demonstrated high reproducibility and discriminating power.
  • Eight distinct substrate profiles were identified among the 30 reference beta-lactamases.
  • The method successfully differentiated between TEM-1, SHV-1, and BRO-1 in clinical isolates.
  • Combined with isoelectric focusing, it offers reliable beta-lactamase identification.

Conclusions:

  • The developed disc diffusion method is a valuable tool for beta-lactamase screening.
  • It aids in epidemiological studies by efficiently characterizing enzyme profiles.
  • The method provides a reliable approach for identifying common beta-lactamases in clinical settings.