Evaluation of three growth factors in combinations of two for temporomandibular joint disc tissue engineering

Tissue engineering of the temporomandibular joint disc could be a great value in treatments that require discectomy. Potential benefit has been found in the use of three growth factors: insulin-like growth factor-I, basic fibroblast growth factor and transforming growth factor-beta1 in maintaining disc-like tissue in culture. In the present study, these three growth factors were combined in pairs and tested at two different concentrations over a 6-week period. All combinations of the growth factors appear to be beneficial since only three-quarters of the control constructs (without growth factors) retained mechanical integrity, compared with the majority of constructs exposed to growth factors. Importantly, the concentrations of the presented growth factors had a significant impact on the cellularity of constructs at Week 6. When a high concentration of the two growth factors was used, at least twice as many cells remained in the constructs compared with controls.