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[Isolation and identification of taxane from Taxus chinensis cell cultures]
1College of Chemistry and Life Sciences, South-Central University for Nationalities, Wuhan.
Objective:
To study taxanes of the cell cultures of Taxus chinensis.
Methods:
The cell cultures were extracted with 95% alcohol, distributed by different solvents, isolated via column chromatogaphy on silica gel by eluting with various proportions of petroleum ether-ethyl acetate and purified by crystallization with ethanol.
Result:
A taxane was isolated from the cell cultures of Taxus chinensis and its structure was identified as 2alpha,5alpha, 10beta, 14beta-tetraacetoxy-taxa-4 (20), 11-diene (Taxuyunnanine C) on the base of spectrum data.
Conclusion:
The compound was isolated from the cell cultures of Taxus chinensis for the first time.
