Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural characterization of the histone variant macroH2A.

Srinivas Chakravarthy1, Sampath Kumar Y Gundimella, Cecile Caron

  • 1Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, 80523-1870, USA.

Molecular and Cellular Biology
|August 19, 2005
PubMed
Summary

MacroH2A, a unique histone variant, possesses an unusual structure impacting nucleosome function. Its nonhistone region associates with histone deacetylases, influencing chromatin acetylation and structure.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PARP1 Exhibits an Enzymatically Inactive Chromatin Binding Mode.

bioRxiv : the preprint server for biology·2026
Same author

Chronic intermittent hypoxia triggers hepatic platelet recruitment.

Sleep medicine·2026
Same author

Predicting isocitrate dehydrogenase mutation status in acute myeloid leukemia from gene expression profiles by machine learning.

NAR genomics and bioinformatics·2026
Same author

Structure of the human HIRA histone chaperone with a nucleosome suggests a stepwise nucleosome assembly mechanism.

bioRxiv : the preprint server for biology·2026
Same author

TFAM organizes DNA into compact higher order structures.

bioRxiv : the preprint server for biology·2026
Same author

Histone diversity in the archaeal domain of life.

Nature communications·2026

Area of Science:

  • Chromatin biology
  • Molecular genetics
  • Structural biology

Background:

  • Histones are core components of chromatin, organizing DNA.
  • Histone H2A has variants, including macroH2A, with unique structures.
  • The function of macroH2A in chromatin regulation is not fully understood.

Purpose of the Study:

  • To investigate the structural organization of macroH2A.
  • To determine how macroH2A influences nucleosome structure and function.
  • To explore the role of the macroH2A nonhistone region in chromatin modification.

Main Methods:

  • X-ray crystallography to determine the structure of the macroH2A nonhistone region.
  • Biochemical assays to study the impact of macroH2A structural changes on nucleosomes.

Related Experiment Videos

  • Analysis of macroH2A association with histone deacetylases.

    • Main Results:

    • macroH2A exhibits an unusual domain structure with a C-terminal nonhistone region linked to the N-terminal histone domain.
    • Alterations in the L1 loop of the histone fold region affect nucleosome structure and function.
    • The nonhistone region adopts an alpha/beta fold and interacts with histone deacetylases.
    • macroH2A influences the acetylation status of its associated nucleosomes.

    Conclusions:

    • The unique domain structure of macroH2A integrates distinct functions.
    • macroH2A plays a crucial role in establishing unique chromatin domains.
    • Structural and functional studies of macroH2A provide insights into chromatin regulation.