Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Red/ET recombination and its biomedical applications].

Jun-Ping Wang1, You-Ming Zhang

  • 1State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, The Third Military Medical University, Chongqing 400038, China. wangjunp@yahoo.com

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|August 20, 2005
PubMed
Summary

Red/ET recombination offers precise DNA engineering for gene deletion, insertion, and mutation. This powerful homologous recombination system has broad biomedical applications and has been recently enhanced for greater maneuverability.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ofatumumab therapy modulates the meningeal lymphatic draining function in patients with multiple sclerosis: a pilot study.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·2026
Same author

Development of a High-Throughput LC-MS/MS Method for Simultaneous Quantification of Four Therapeutic Monoclonal Antibodies in Human Serum: Application in Clinical Therapeutic Drug Monitoring.

Biomedical chromatography : BMC·2026
Same author

Cedrol ameliorates ulcerative colitis <i>via</i> myeloid differentiation factor 2-mediated inflammation suppression, with barrier restoration and microbiota modulation.

World journal of gastroenterology·2026
Same author

Treatment strategy for myasthenia gravis with GAD65-IgG associated neurological disorders: A case report.

Journal of neuroimmunology·2025
Same author

GutGPT: A multidimensional knowledge-enhanced large language model for gastrointestinal medicine.

Journal of biomedical informatics·2025
Same author

Multi-omics reveals the associations among the fecal metabolome, intestinal bacteria, and serum indicators in patients with hepatocellular carcinoma.

World journal of gastroenterology·2025

Area of Science:

  • Molecular Biology
  • Genetic Engineering
  • Biotechnology

Background:

  • Homologous recombination is crucial for DNA manipulation.
  • Bacteriophage-derived systems offer efficient DNA engineering tools.
  • The Red/ET recombination system provides a robust platform for genetic modification.

Purpose of the Study:

  • To review the characteristics and development of Red/ET recombination.
  • To highlight the biomedical applications of Red/ET recombination.
  • To discuss recent improvements in Red/ET recombination technology.

Main Methods:

  • Utilizes Red operon from lambda phage or RecE/RecT from Rac phage.
  • Employs PCR-derived DNA fragments or oligonucleotides for recombination.
  • Facilitates gene deletion, insertion, and mutation with high precision.

Related Experiment Videos

Main Results:

  • Red/ET recombination enables rapid and precise genetic modifications.
  • Applications include bacterial artificial chromosome modification and gene knock-out construction.
  • The system is applicable to E. coli and other microorganisms.

Conclusions:

  • Red/ET recombination is a versatile and powerful tool for DNA engineering.
  • Recent advancements have increased its efficiency and maneuverability.
  • It holds significant potential for diverse biomedical research and applications.