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Cloning and nucleotide sequences of two lipase genes from Candida cylindracea
S Longhi1, F Fusetti, R Grandori
1Dipartimento di Fisiologia e Biochimica Generali, Università degli Studi di Milano, Italy.
Biochimica Et Biophysica Acta
|June 15, 1992
Abstract:
Two lipase-encoding genes (LIP1 and LIP2) have been isolated from a SacI genomic library of the yeast Candida cylindracea and their nucleotide sequences have been determined. Comparison with the sequence of a cDNA ruled out the presence of introns in the two genes. Both ORFs encode for mature proteins of 534 residues with putative signal peptides of 15 and 14 amino acids, respectively. When compared with other lipase sequences, the two C. cylindracea lipases showed homology only with the Geotrichum candidum lipase, whereas they shared a significant similarity with several esterases.