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Related Concept Videos

DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Tissue Homogenization and Cell Lysis01:32

Tissue Homogenization and Cell Lysis

Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...

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Related Experiment Video

Updated: Jun 25, 2026

Detection of Histone Modifications in Plant Leaves
07:08

Detection of Histone Modifications in Plant Leaves

Published on: September 23, 2011

Rapid and simple procedure for homogenizing leaf tissues suitable for mini-midi-scale DNA extraction in rice.

Gihwan Yi1, Jun-Ho Choi, Jong-Hee Lee

  • 1Rice Division, Yeongnam Agricultural Research Institute, Milyang, Korea. gihwan@rda.go.kr

Preparative Biochemistry & Biotechnology
|August 20, 2005
PubMed
Summary
This summary is machine-generated.

This study introduces a fast and easy method for grinding rice leaf samples using tungsten carbide beads and a vortexer. This technique efficiently prepares samples for DNA extraction, yielding high-quality DNA suitable for PCR and RFLP analysis.

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Area of Science:

  • Plant molecular biology
  • Agricultural biotechnology
  • Genomics research

Background:

  • Efficient DNA extraction is crucial for molecular biology techniques in rice.
  • Existing methods for leaf sample homogenization can be time-consuming or require specialized equipment.
  • Standardized and rapid protocols are needed for high-throughput genetic analysis in rice.

Purpose of the Study:

  • To develop and present a rapid and simple procedure for homogenizing rice leaf samples.
  • To optimize sample preparation for mini/midi-scale DNA extraction.
  • To facilitate downstream molecular analyses such as PCR and RFLP.

Main Methods:

  • Utilized tungsten carbide beads for mechanical disruption of leaf tissues.
  • Employed a standard vortexer with a specialized attachment for simultaneous grinding of multiple samples.
  • Optimized grinding time for efficient sample homogenization.

Main Results:

  • Achieved complete homogenization of two rice leaf samples within 11.3 ± 1.5 seconds.
  • Demonstrated the capacity to process up to 20 samples concurrently using the vortexer attachment.
  • Obtained DNA yields ranging from 2.2 to 7.6 µg from 25-150 mg of fresh leaf tissue.
  • Confirmed DNA quality and quantity suitable for Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) analyses.

Conclusions:

  • The described method offers a rapid, simple, and efficient approach for rice leaf sample homogenization.
  • This protocol is compatible with mini/midi-scale DNA preparation, supporting various molecular applications.
  • The technique enhances throughput for genetic studies in rice, enabling broader genomic research.