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Related Experiment Videos

Using multilocus sequence data to define the pneumococcus.

William P Hanage1, Tarja Kaijalainen, Elja Herva

  • 1Department of Infectious Disease Epidemiology, St. Mary's Hospital, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom. w.hanage@imperial.ac.uk.

Journal of Bacteriology
|August 20, 2005
PubMed
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Genetic analysis revealed distinct groups within nonserotypeable pneumococci. Some are authentic pneumococci, while others represent a related but separate bacterial population, challenging traditional identification methods.

Area of Science:

  • Microbiology
  • Genetics
  • Bacterial Taxonomy

Background:

  • Serotypeable pneumococci are well-characterized pathogens.
  • Nonserotypeable presumptive pneumococci pose diagnostic challenges.
  • Distinguishing authentic pneumococci from related bacteria is crucial for accurate identification.

Purpose of the Study:

  • To genetically differentiate serotypeable and nonserotypeable pneumococci.
  • To identify distinct bacterial populations within nonserotypeable isolates.
  • To evaluate multilocus sequence typing (MLST) and pneumolysin gene (ply) sequencing for bacterial classification.

Main Methods:

  • Multilocus sequence typing (MLST) was performed on 121 nonserotypeable presumptive pneumococci.
  • Partial sequencing of the pneumolysin gene (ply) was utilized.

Related Experiment Videos

  • Phylogenetic analysis based on concatenated MLST sequences was conducted.

    • Main Results:

    • Three distinct groups of nonserotypeable isolates were identified.
    • Group 1: Authentic pneumococci with downregulated or lost capsular expression.
    • Group 2: Closely related to pneumococci but with distinct MLST sequence types (STs).
    • Group 3: Genetically divergent from pneumococci, with distinct ply alleles.
    • Recombination between pneumococci and the related population was observed.
    • MLST phylogenetic trees generally distinguished between pneumococci and the related population.
    • Ply gene sequencing aided in resolving difficult cases.

    Conclusions:

    • Nonserotypeable isolates include both authentic pneumococci and a distinct, related bacterial population.
    • Traditional identification methods like optochin susceptibility are insufficient for complete resolution.
    • MLST and ply gene sequencing are valuable tools for bacterial classification and resolving taxonomic ambiguities.
    • Accurate bacterial identification is essential for understanding pneumococcal epidemiology and disease control.