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Updated: Aug 10, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
Analysis of mitogen-activated protein kinase activation
1Cardiovascular Research Institute, University of South Dakota School of Medicine, Sioux Falls, SD, USA.
Abstract:
The biological functions of mast cells are regulated by several protein kinases, including the tyrosine kinases Fyn, Lyn, Syk, and FAK and the serine/threonine kinases Akt and PKC alpha/beta. The mitogen-activated protein kinases extracellular signal-regulated kinases, JNK, and p38MAPK also play a significant role in the regulation of mast cell biological function. This chapter will detail recent advances in determining mitogen-activated protein kinase activation in single cells. These methods are applicable to studies of signal transduction in mast cells.
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