Glial fibrillary acidic protein and vimentin expression in the frog olfactory system during metamorphosis

In the present study, we investigated glial cell organization in the olfactory system of adult and tadpole Xenopus laevis using glial fibrillary acidic protein and vimentin antibodies. Our results showed for the first time that glial fibrillary acidic protein was strongly expressed at the level of the olfactory nerve from tadpole to adult and was likely to be expressed by ensheathing glia. In the olfactory bulb, the nerve layer was stained, and no staining was observed in glomeruli. By contrast, vimentin decorated radial glia in the bulb but faintly stained the olfactory nerve. Interestingly, glial fibrillary acidic protein and vimentin presented complementary staining patterns, with glial fibrillary acidic protein being expressed in the peripheral olfactory system and vimentin being expressed in the central part of the olfactory system.