[Effects of culture condition on fatty acids in Pseudcarbomonas sp]

The investigation on the growth of Pseudomonas sp. and the dynamics of its fatty acids under various culture conditions indicated that the optimum temperature for its growth was 30 degrees C, and the best carbon source was starch. 13 kinds of fatty acids were detected, among which, c16:1(n-7), c15:0, c16:0, c17:0, c18:1(n-6), c18:1 (n-9), 9, 10-cp-c17:0 and iso9, 10-cp-c17:0 were the major ones. When cultured at 30 degrees C, the relative content of unsaturated fatty acids decreased significantly. Carbon source in the media was the crucial factor affecting the contents of odd- and cyclopropane fatty acids. Cluster analysis revealed that temperature had a more obvious effect than carbon source.