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Related Experiment Videos

Circulating stem cells in mice treated with cyclophosphamide.

C F Craddock1, J F Apperley, E G Wright

  • 1Leukaemia Research Fund Centre, Institute of Cancer Research, London, United Kingdom.

Blood
|July 1, 1992
PubMed
Summary
This summary is machine-generated.

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Cyclophosphamide (CY) chemotherapy mobilizes hematopoietic stem cells in mice, enabling peripheral blood collection for potential transplantation. These cells rescued lethally irradiated mice and showed long-term proliferation without inducing leukemia.

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Cancer Research

Background:

  • Chemotherapy is used to mobilize hematopoietic progenitor cells for autologous transplantation.
  • Clinical monitoring relies on circulating CFU-GM and CD34+ cells, but marrow-repopulating stem cells remain unconfirmed.
  • Previous studies lacked definitive proof of marrow-repopulating stem cells post-chemotherapy mobilization.

Purpose of the Study:

  • To investigate the efficacy of cyclophosphamide (CY) in mobilizing marrow-repopulating stem cells in mice.
  • To confirm the presence and function of mobilized stem cells in peripheral blood after CY treatment.
  • To assess the leukemogenic potential of CY-mobilized cells and their response to irradiation.

Main Methods:

  • Mice were treated with 200 mg/kg cyclophosphamide (CY).

Related Experiment Videos

  • Peripheral blood was analyzed for white blood cells, CFU-S12, and CFU-GM counts.
  • Mobilized cells were used to rescue lethally irradiated, sex-mismatched recipient mice.
  • In vitro culture with IL-3 and assessment of leukemogenic potential post-irradiation were performed.

    • Main Results:

    • Peak numbers of CFU-S12 and CFU-GM were observed 8 days post-CY treatment.
    • Peripheral blood cells harvested at peak times rescued lethally irradiated mice, with confirmed donor cell engraftment.
    • In vitro cultured cells proliferated for over a year but lost rescue/colony-forming abilities without becoming leukemic.
    • CY-treated mice irradiated at peak cell counts did not show increased leukemia incidence compared to controls.

    Conclusions:

    • Cyclophosphamide effectively mobilizes functional, marrow-repopulating stem cells into the mouse peripheral blood.
    • Mobilized stem cells can be cultured long-term but lose some functions over time.
    • CY-induced stem cell mobilization does not increase the risk of developing leukemia, even with subsequent irradiation.