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Determination of leflunomide in tablets by high performance liquid chromatography
Duygu Yeniceli1, Dilek Dogrukol-Ak, Muzaffer Tuncel
1Department of Analytical Chemistry, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey.
Abstract:
In the present study, a reverse phase high performance liquid chromatography (HPLC) method was validated and applied for the determination of leflunomide in tablets. Chromatographic separation of leflunomide and oxazepam as an internal standard was carried out on a C(18) column (50 mm, 3 mm i.d.) using a mobile phase, consisting of methanol and water (60:40, v/v), at a flow rate of 0.5 ml min(-1) and UV detection at 260 nm. The retention times for oxazepam and leflunomide were 2.6 and 5.2 min, respectively. The validated quantification range of the method was 2.7 x 10(-6) to 5.5 x 10(-5) M for leflunomide. The results of the developed procedure in tablets were compared with those of UV spectrophotometry to assess active leflunomide content.
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