Related Experiment Videos
Presence and specific concentration of carbonic anhydrase II in matrix vesicles
D J Stechschulte1, D C Morris, S F Silverton
1Department of Pathology, University of Kansas Medical Center, Kansas City.
Bone and Mineral
|May 11, 1992
Abstract:
Matrix vesicles were isolated from the epiphyseal growth plates of normal weanling rats, and the presence of carbonic anhydrase II was demonstrated by Western blotting and ultrastructural immunolocalization using the immunogold technique. Total carbonic anhydrase activity was assayed and showed a statistically significant increase in matrix vesicles as compared to normal rat chondrocytes derived from the same growth plates. These results are the first to establish the presence of carbonic anhydrase in matrix vesicles.