Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mixed phospholipid liposome calcification.

E D Eanes1

  • 1Bone Research Branch Research Associate Program, National Institute of Dental Research, Gaithersburg, Maryland 20899.

Bone and Mineral
|May 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Amorphous Calcium Phosphate-Based Bioactive Polymeric Composites for Mineralized Tissue Regeneration.

Journal of research of the National Institute of Standards and Technology·2016
Same author

Dental composites based on hybrid and surface-modified amorphous calcium phosphates.

Biomaterials·2003
Same author

Amorphous calcium phosphate.

Monographs in oral science·2002
Same author

Role of OCP in biological processes.

Monographs in oral science·2002
Same author

Silica- and zirconia-hybridized amorphous calcium phosphate: effect on transformation to hydroxyapatite.

Journal of biomedical materials research·2002
Same author

Physicochemical evaluation of bioactive polymeric composites based on hybrid amorphous calcium phosphates.

Journal of biomedical materials research·2000

Synthetic lipid vesicles (liposomes) mimic matrix vesicle calcification. Specific phospholipids like phosphatidylserine and sphingomyelin in liposomes slow calcium phosphate precipitation, offering insights into bone mineralization.

Area of Science:

  • Biochemistry
  • Biomineralization
  • Lipid membrane studies

Background:

  • Synthetic lipid vesicles (liposomes) serve as experimental models for matrix vesicle (MV) calcification.
  • Precipitate formation in liposomes can mimic the intraliposomal and subsequent extracellular progression observed in MV calcification.

Purpose of the Study:

  • To investigate how specific phospholipid (PL) components within liposomal membranes influence calcium phosphate precipitation.
  • To determine the role of MV-enriched lipids in modulating precipitation progression.

Main Methods:

  • Utilizing synthetic lipid vesicle (liposome) suspensions to model calcium phosphate precipitation.
  • Analyzing the effect of different phospholipid constituents on the spatial and temporal progression of precipitation.

Related Experiment Videos

Main Results:

  • Certain phospholipid constituents of liposomes modulate calcium phosphate precipitation progression.
  • Phosphatidylserine and sphingomyelin, lipids enriched in MVs, significantly slow the outward expansion of precipitation from within liposomes.

Conclusions:

  • Phospholipid composition of membranes plays a critical role in regulating biomineralization processes.
  • The findings provide a mechanistic understanding of how MV-enriched lipids influence the initial stages of calcification, relevant to bone formation.