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Published on: January 17, 2014
Analysis of cellular phenotypes that mediate genetic resistance to tuberculosis using a radiation bone marrow chimera
Konstantin B Majorov1, Evgeny B Eruslanov, Elvira I Rubakova
1Laboratory for Immunogenetics, Central Institute for Tuberculosis, Yauza alley 2, Moscow 107564, Russia.
Adoptive transfer of bone marrow cells from tuberculosis-resistant (I/St x A/Sn)F(1) donor mice into lethally irradiated susceptible I/St recipients changed their phenotype following infection with virulent Mycobacterium tuberculosis. Compared to I/St-->I/St control animals, F(1)-->I/St chimeras demonstrated (i) prolonged survival time, (ii) increased antimycobacterial function of lung macrophages, (iii) elevated gamma interferon production by lung cells, and (iv) decreased infiltration of the lungs with CD4(+) and CD8(+) T cells and Ly-6G(+) neutrophils.
Adoptive transfer of bone marrow cells from tuberculosis-resistant (I/St x A/Sn)F(1) donor mice into lethally irradiated susceptible I/St recipients changed their phenotype following infection with virulent Mycobacterium tuberculosis. Compared to I/St-->I/St control animals, F(1)-->I/St chimeras demonstrated (i) prolonged survival time, (ii) increased antimycobacterial function of lung macrophages, (iii) elevated gamma interferon production by lung cells, and (iv) decreased infiltration of the lungs with CD4(+) and CD8(+) T cells and Ly-6G(+) neutrophils.
