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Identification of Functional Protein Regions Through Chimeric Protein Construction
Published on: January 8, 2019
Chimeric structure of omp2 of Brucella from Pacific common minke whales (Balaenoptera acutorostrata)
Kazue Ohishi1, Kiyotaka Takishita, Masaru Kawato
1Research Program for Marine Biology and Ecology, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Kanagawa, Japan. oishik@jamstec.go.jp
Abstract:
In the Pacific common minke whale (Balaenoptera acutorostrata ), a new variant of Brucella has been detected using the polymerase chain reaction. Detailed analysis of the porin protein genes, omp2a and omp2b from the whale Brucella showed that these two genes have some motifs in common with Atlantic marine strains in the 5'-terminal one-third region. On the other hand, the nucleotide sequences in the 3'-terminal two-thirds region of the two genes were almost identical to the respective genes of terrestrial strains. Thus, Pacific whale Brucella omp2 genes are chimeras between marine and terrestrial strains.
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