Few polyploid blastomeres in morphologically superior bovine embryos produced in vitro

Morphologically inferior bovine embryos developed in vivo have been shown by karyotyping to have a higher rate of chromosomally abnormal cells than morphologically normal embryos. The objective of this study was to re-examine this finding using interphase cytogenetics. A total of 155 IVP Day 8 bovine blastocysts were graded by their morphology (excellent, good, or poor) and timing of development (hatched, expanded, or non-expanded), and afterwards analysed for chromosome abnormalities by fluorescence in situ hybridization using differentially labelled probes for chromosomes 6 and 7. The overall frequency of diploid embryos was 7%, and did not differ according to grading. Although the frequency of mixoploidy was not correlated to the morphological grading, the blastocysts with excellent morphology displayed fewer polyploid nuclei in comparison to blastocysts with good (P=0.05) or poor morphology (P=0.01). There were however also prominent exceptions showing that a blastocyst with an excellent morphology can display a high degree of polyploidy. The results further demonstrate that the morphologically normal embryos contain a higher number of cells and develop more rapidly than the morphologically inferior embryos.