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Updated: Aug 13, 2026

An In Ovo Model for Testing Insulin-mimetic Compounds
Published on: April 23, 2018
An anionic inositol phosphate glycan pseudotetrasaccharide exhibits high insulin-mimetic activity in rat adipocytes
Nilanjana Chakraborty1, Marc d'Alarcao
1Michael Chemistry Laboratory, Department of Chemistry, Tufts University, Medford, MA 02155, USA.
Abstract:
Inositol phosphate glycan pseudotetrasaccharides consisting of man-(alpha1-6)-man-(alpha1-4)-glcN-(alpha,beta1-6)-myo-inositol-1,2-cyclic phosphate possessing a sulfate group at either O-6 (compounds 3alpha,beta) or O-2 (compounds 4alpha,beta) of the terminal mannose have been prepared. Compound 4alpha was able to stimulate lipogenesis in native rat adipocytes to 78% of the maximal insulin response (MIR) with an EC50 of 1.1 microM. The other compounds exhibited lower maximal stimulations (47-63% MIR) and higher EC50 values (9.5-10.6 microM).
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