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Rapid and simple isolation of DNA from agarose gels
1Institut für Mikrobiologie, J. W. Goethe-Universität, Frankfurt/Main, Federal Republic of Germany.
Current Genetics
|July 1, 1992
Abstract:
We present a simple method for the isolation of DNA from agarose gels that is economic, fast, and independent of electrical equipment. DNA fragments of up to 6 kb can be easily extracted within 5 min using a disposable plastic syringe and filter paper. Total extraction of DNA fragments between 10 and 20 kb in size is achieved by concentrating the DNA flushed from the gel in a DNA-binding column.