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Published on: February 28, 2015
Single-walled carbon nanotube biosensors using aptamers as molecular recognition elements
Hye-Mi So1, Keehoon Won, Yong Hwan Kim
1Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 305-343, Korea.
Journal of the American Chemical Society
|August 25, 2005
Summary
This study demonstrates real-time protein detection using DNA aptamers on single-walled carbon nanotube field-effect transistors (SWNT-FETs). The biosensor accurately identifies thrombin, showing a significant change in electrical conductance upon binding.
Area of Science:
- Nanotechnology
- Biotechnology
- Biochemistry
Background:
- Protein detection is crucial for diagnostics.
- Existing methods can be time-consuming or require complex sample preparation.
- Development of rapid, sensitive biosensors is needed.
Purpose of the Study:
- To develop a real-time protein detection system using single-walled carbon nanotube field-effect transistors (SWNT-FETs).
- To utilize DNA aptamers as specific molecular recognition elements for target proteins.
- To investigate the sensor's response to thrombin and a control protein.
Main Methods:
- Immobilization of anti-thrombin DNA aptamers onto SWNT-FETs using CDI-Tween linkers.
- Monitoring changes in threshold gate voltage and conductance of the SWNT-FET.
- Introducing thrombin and elastase solutions to assess sensor specificity and response.
Main Results:
- The binding of thrombin to immobilized aptamers caused a rightward shift in threshold gate voltage.
- A significant decrease in conductance was observed upon thrombin addition.
- No noticeable change in conductance was detected when elastase was introduced, indicating specificity.
Conclusions:
- SWNT-FETs functionalized with DNA aptamers enable real-time, specific detection of proteins.
- This aptasensor shows promise for sensitive and rapid protein analysis.
- The sensor's electrical response is directly correlated with target protein binding.
