Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

¹H NMR: Complex Splitting

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.

Spectrophotometry: Introduction

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...

Interaction of EM Radiation with Matter: Spectroscopy

Interaction of EM Radiation with Matter: Spectroscopy

Electromagnetic (EM) radiation can be considered an oscillating electric and magnetic field propagating through a medium that can interact with matter in its path. The electric field in the radiation can interact with electrical charges in the atoms or molecules in the matter. On the other hand, the magnetic field can interact with the magnetic field in the atomic nucleus. The study of the interaction between electromagnetic radiation and matter is termed spectroscopy. Spectroscopy is the study...

Molecular Spectroscopy: Absorption and Emission

Molecular Spectroscopy: Absorption and Emission

Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels. Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.

UV–Vis Spectrometers

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Anharmonic Effects Revealed by Temperature-Dependent Phonon Lifetimes and Thermal Conductivities in a π-Conjugated Molecular Crystal.

Journal of chemical theory and computation·2026

Same author

Ion Transport through Permselective Nanochannels of 2D-Covalent Organic Frameworks: Insights from Nonequilibrium Atomistic MD Simulations.

The journal of physical chemistry. B·2026

Same author

Lanthanide-doped nanocrystals enable organic room-temperature phosphorescence in solution through direct triplet excitation.

Nature chemistry·2026

Same author

Fully Textured Monolithic Sb<sub>2</sub>S<sub>3</sub>/Silicon Tandem for Unbiased and Stable Solar-Driven Water Splitting Paired with Iodide Oxidation Reaction.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Coulombic control of charge transfer in radicals with quartet recycling luminescence.

Nature communications·2026

Same author

On the Design of Steep Optical Absorbers for Vacuum-Processed Organic Solar Cells: One Isopropyl Group Makes the Difference.

Small science·2026

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

Single molecule spectroscopy as a probe for dye-polymer interactions.

Renaud A L Vallée¹, Philippe Marsal, Els Braeken

¹Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, B-3001 Leuven, Belgium. renaud.vallee@chem.kuleuven.be

Journal of the American Chemical Society

|August 25, 2005

Summary

This study reveals polymer-dye molecules adopt planar or nonplanar shapes within polystyrene. Planar forms further classify based on side-chain interactions, offering insights into polymer dynamics.

More Related Videos

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Related Experiment Videos

Last Updated: Jul 5, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Area of Science:

Polymer Science
Spectroscopy
Computational Chemistry

Background:

Understanding polymer-dye interactions is crucial for material science.
Molecular conformations influence material properties.

Purpose of the Study:

Investigate the behavior and dynamics of a specific polymer-dye system.
Characterize dye molecule conformations within a polymer matrix.

Main Methods:

Combined experimental (Single Molecule Spectroscopy) and theoretical approaches.
Utilized quantum-chemical calculations, Monte Carlo, and molecular dynamics simulations.

Main Results:

Identified two primary dye molecule conformations: planar and nonplanar.
Classified planar conformers based on alkyl side-chain interactions with the polymer matrix.
Analyzed fluorescence lifetime trajectories to distinguish conformations.

Conclusions:

Polymer-dye molecule conformation is dependent on the surrounding matrix.
Side-chain interactions play a key role in defining dye molecule behavior within polymers.
This research provides a detailed understanding of molecular dynamics in polymer systems.