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Related Concept Videos

RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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Types of RNA01:20

Types of RNA

9.1K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

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PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
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Related Experiment Video

Updated: Jan 19, 2026

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
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RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

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[Double stranded RNA (RNA interference RNAi) in different creatures].

Bei Han1, Xiu-Min Wang, Xue-Fan Gu

  • 1Molecular Biology Laboratory of Xinhua Hospital Shanghai 200092, China. betty_han@sina.com

Yi Chuan = Hereditas
|August 25, 2005
PubMed
Summary

Double stranded RNA (dsRNA) effectively degrades target gene mRNA, aiding gene function studies across diverse organisms like Arabidopsis, C. elegans, Drosophila, zebrafish, and mice. Recent applications of dsRNA are highlighted.

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Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
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Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
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RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
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Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Context:

  • Gene silencing is crucial for understanding gene function.
  • Double-stranded RNA (dsRNA) has emerged as a powerful tool for targeted gene knockdown.
  • Its application spans various model organisms, including plants, nematodes, insects, and vertebrates.

Purpose:

  • To review the recent applications of dsRNA technology in biological research.
  • To highlight the versatility and efficacy of dsRNA for studying gene function.
  • To provide an overview of dsRNA's utility across different species.

Summary:

  • Double-stranded RNA (dsRNA) mediates the degradation of specific messenger RNA (mRNA) molecules, leading to gene silencing.
  • This mechanism is widely employed in diverse model organisms such as Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish, and Mouse.
  • Recent advancements have expanded the applications of dsRNA in functional genomics and molecular studies.

Impact:

  • Facilitates rapid and specific investigation of gene function.
  • Enables the study of gene roles in complex biological processes.
  • Provides a valuable tool for research in developmental biology, disease modeling, and biotechnology.