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Related Concept Videos

What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...

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Related Experiment Video

Updated: Jun 28, 2026

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
09:13

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

[Serial analysis of gene expression].

Ji Huang1, Hong-Sheng Zhang, Dong Wang

  • 1National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agri. Univ., Nanjing 210095, China. rice-hj@263.net

Yi Chuan = Hereditas
|August 25, 2005
PubMed
Summary
This summary is machine-generated.

Serial analysis of gene expression (SAGE) is a sequencing technique for detailed gene expression profiling. It aids in transcriptome research and identifying gene expression differences in various tissues.

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Last Updated: Jun 28, 2026

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
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Published on: January 13, 2016

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
09:45

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Published on: August 10, 2017

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Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress

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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Context:

  • Gene expression analysis is crucial for understanding cellular function and disease.
  • Existing methods for gene expression profiling have limitations in comprehensiveness and quantification.

Purpose:

  • To review the principles, development, and applications of Serial Analysis of Gene Expression (SAGE).
  • To highlight SAGE's utility in transcriptome research and differential gene expression analysis.

Summary:

  • Serial Analysis of Gene Expression (SAGE) is a sequencing-based technique enabling comprehensive and quantitative gene expression profiling.
  • SAGE analyzes short sequence tags to represent mRNA populations from specific tissues or cells.
  • The technique has been successfully applied to transcriptome research and identifying differentially expressed genes.

Impact:

  • SAGE provides a powerful tool for researchers studying gene expression patterns.
  • Facilitates the discovery of novel genes and biomarkers.
  • Contributes to a deeper understanding of biological processes and disease mechanisms.