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Published on: May 22, 2012
Improved PCR method for amplification of GC-rich DNA sequences
F Hubé1, P Reverdiau, S Iochmann
1Inserm, U618, Tours, F-37032 France; IFR 135, Tours, F-37044 France.
Abstract:
Most housekeeping genes, tumor-suppressor genes, and approx 40% of tissue-specific genes contain G+C sequences in their promoter region that were very difficult to amplify. In this report, we propose an improved polymerase chain reaction (PCR) method to be used for successful amplification of the tissue factor pathway inhibitor (TFPI)-2 gene promoter region that exhibit >70% G+C content in a sequence of approx 300 bp and a complete CpG island region spanning exon 1, the three transcription initiation sites, and the translation start site. Therefore, this method can be recommended to amplify other GC-rich genomic templates.
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