Related Experiment Videos
Immunochemical studies of histone H5 from Halobatrachus didactylus
C Pendon1, M C Rendon, M C Sarrasquete
1Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad de Cadiz, Puerto Real, Spain.
Abstract:
1. Histone H5 from Halobatrachus didactylus was isolated by using perchloric acid (PCA) extraction of fish liver nuclei and trichloroacetic acid (TCA) precipitation. 2. A polyclonal antiserum was generated by immunizing rabbits with the antigen purified from SDS-PAGE. 3. By immunofluorescence the serum stains erythrocyte nuclei from H. didactylus but it does not react with mammalian cells. 4. By Western blotting, the anti-H5 antibody reacts with the isolated antigen at high titers. 5. Digestion of histone H5 with pepsin and cyanogen bromide suggests that the epitopes are located in the globular and C-terminal region of the H5 molecule excluding the N-terminal.