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Updated: Aug 16, 2026

Generation and Culturing of Primary Human Keratinocytes from Adult Skin
Published on: December 22, 2017
Interleukin-19 upregulates keratinocyte growth factor and is associated with psoriasis
Hsing-Hui Li1, Y-C Lin, P-J Chen
1Institute of Basic Medical Sciences, Medical College, National Cheng Kung University, no. 1 Ta-Hsueh Road, Tainan 704, Taiwan.
Background:
Interleukin (IL)-19, a member of the IL-10 family, signals through the IL-20R1/IL-20R2 heterodimer, which is shown to be involved in abnormal keratinocyte differentiation and proliferation. Little is known about its in vitro biological functions or its role in psoriasis.
Objectives:
To investigate the role of IL-19 in the psoriatic process.
Methods:
The expression of keratinocyte growth factor (KGF) transcripts was measured by polymerase chain reaction in CD8+ T cells treated with IL-19. Next, we developed monoclonal and polyclonal antibodies to measure the levels of IL-19 in the sera of patients with psoriasis and healthy volunteers using an enzyme-linked immunosorbent assay. In addition, we performed immunohistochemical staining on psoriatic skin and normal controls.
Results:
We found that IL-19 upregulated KGF transcripts on CD8+ T cells. Patients with psoriasis had a lower level of IL-19 in serum than healthy volunteers. The difference between these two groups was statistically significant (P < 0.05). IL-19 expression was seen in basal and suprabasal keratinocytes in a continuous pattern, and was increased in psoriatic epidermis.
Conclusions:
These results suggest that IL-19 plays a role in the complex pathological cytokine network in psoriasis.
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