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Updated: Jul 25, 2026
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
Published on: October 30, 2015
Higher respiratory rates and improved creatine stimulation in brain mitochondria isolated with anti-oxidants
G J Brewer1, T T Jones, T Wallimann
1Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, P.O. Box 19626, Springfield, IL 62794-9230, USA. gbrewer@siumed.edu
Abstract:
We tested the effect of an anti-oxidant mixture on respiration in isolated rat brain mitochondria. Mitochondria were isolated in mannitol/sucrose/EGTA/BSA +/- SCAVEGR anti-oxidants (SOD, catalase, vitamin E, vitamin E acetate, and glutathione reduced). TBARS were reduced by greater than 40% with SCAVEGR. Respiration driven by ADP showed a two-fold higher V(max) and a 15% higher respiratory control ratio when mitochondria were prepared with SCAVEGR. SCAVEGR also stabilized the octameric state of mitochondrial creatine kinase and thus improved creatine-stimulated respiration. These results suggest that significant improvements in brain mitochondrial function are obtained by isolation in the presence of an anti-oxidants mixture.
