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Related Experiment Videos

Human UDP-glucuronosyltransferase 1A5: identification, expression, and activity.

Moshe Finel1, Xin Li, Dione Gardner-Stephen

  • 1Viikki DDTC, Faculty of Pharmacy, University of Helsinki, P.O. Box 56 (Viikinkaari 5E), 00014 University of Helsinki, Finland. moshe.finel@helsinki.fi

The Journal of Pharmacology and Experimental Therapeutics
|August 27, 2005
PubMed
Summary

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This study confirms human UDP-glucuronosyltransferase 1A5 (UGT1A5) is expressed in various tissues, is inducible by drugs, and shows catalytic activity. Researchers found UGT1A5 in liver and intestine, with levels varying between individuals.

Area of Science:

  • Pharmacogenomics
  • Drug metabolism
  • Enzymology

Background:

  • The human UDP-glucuronosyltransferase (UGT) subfamily 1A comprises nine genes.
  • Expression of UGT1A isoforms, except UGT1A5, has been previously documented.

Purpose of the Study:

  • To investigate the expression, inducibility, and catalytic activity of the human UGT1A5 gene.
  • To characterize the substrate specificity of the UGT1A5 enzyme.

Main Methods:

  • Detection of UGT1A5 mRNA in cultured human hepatocytes, HepG2, Caco-2 cells, and human liver and intestinal tissues.
  • Induction studies using rifampicin and 3-methylcholanthrene.
  • Isolation of full-length UGT1A5 cDNA and recombinant protein expression.
  • Enzymatic assays using various substrates (4-methylumbelliferone, scopoletin, 1-hydroxypyrene, 4-aminobiphenyl).

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  • Site-directed mutagenesis to investigate substrate binding.

    • Main Results:

    • Low basal UGT1A5 expression detected in cultured human hepatocytes, HepG2, Caco-2 cells, and human liver.
    • UGT1A5 mRNA levels increased upon treatment with rifampicin or 3-methylcholanthrene.
    • UGT1A5 expression found in various human intestinal segments with significant interindividual variability.
    • Recombinant UGT1A5 showed low activity for 4-methylumbelliferone and scopoletin but higher activity for 1-hydroxypyrene.
    • UGT1A5 did not glucuronidate 4-aminobiphenyl; mutagenesis studies did not alter this activity.

    Conclusions:

    • The human UGT1A5 gene is expressed in multiple tissues, including the liver and intestine.
    • UGT1A5 expression is inducible by xenobiotics.
    • The UGT1A5 enzyme is catalytically active, with specific substrate preferences, and plays a role in drug metabolism.