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Local regulatory variation in Saccharomyces cerevisiae
James Ronald1, Rachel B Brem, Jacqueline Whittle
1Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
Plos Genetics
|August 27, 2005
Summary
Genetic variation within yeast (Saccharomyces cerevisiae) significantly impacts gene expression. Nearly a quarter of yeast genes exhibit local regulatory variation, influencing traits and highlighting the role of cis-acting elements.
Area of Science:
- Genetics
- Molecular Biology
- Yeast Genomics
Background:
- Naturally occurring sequence variation influences gene expression and phenotypic diversity.
- Regulatory variation can act locally (cis) or at distant genomic locations (trans).
Purpose of the Study:
- To analyze genome-wide local regulatory variation in Saccharomyces cerevisiae.
- To investigate the mechanisms and extent of cis-acting regulatory variation in yeast.
Main Methods:
- Genetic linkage analysis to identify genes with local regulatory variation.
- Allele-specific expression measurements in a diploid yeast hybrid.
- Genome-wide sequence analysis of promoter and untranslated regions.
Main Results:
- Nearly 25% of yeast genes showed local regulatory variation between divergent strains.
- Local regulatory variation acted in cis for 52%-78% of analyzed genes.
- Increased sequence polymorphism was observed in promoter and 3' UTR regions of affected genes.
Conclusions:
- Cis-acting variation is crucial for regulating gene expression in yeast.
- Local regulatory variation contributes to phenotypic diversity through diverse mechanisms.
- Promoter and 3' UTR sequence polymorphisms are associated with regulatory variation.
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