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Two fish bile reference materials certified for PAH metabolites.
Freek Ariese1, Jonny Beyer, David Wells
1Department of Analytical Chemistry and Applied Spectroscopy & Institute for Environmental Studies, Vrije Universiteit Amsterdam, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands. f.ariese@few.vu.nl
Journal of Environmental Monitoring : JEM
|August 27, 2005
Summary
New certified reference materials for fish bile are now available to validate analytical methods for polycyclic aromatic hydrocarbons (PAHs) and their metabolites, improving environmental monitoring of aquatic ecosystems.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
- Ecotoxicology
Background:
- Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants of concern in aquatic ecosystems.
- PAH metabolites in fish bile serve as biomarkers for exposure assessment.
- Lack of certified reference materials hindered accurate analytical method validation and quality control for PAH metabolites.
Purpose of the Study:
- To prepare and certify two fish bile reference materials for PAH metabolite analysis.
- To provide essential tools for quality control in environmental monitoring of PAH pollution.
Main Methods:
- Preparation and characterization of two fish bile materials (BCR 720 and BCR 721).
- Homogeneity and stability testing of the reference materials.
- Certification measurements by 12 expert laboratories using liquid chromatography with fluorescence detection (LC-FLD) and gas chromatography with mass spectrometry (GC-MS).
Main Results:
- BCR 720 certified for 1-hydroxyphenanthrene, 1-hydroxypyrene, and 3-hydroxybenzo[a]pyrene.
- BCR 721 certified for 1-hydroxypyrene.
- Results for 2-hydroxynaphthalene are listed but not certified.
Conclusions:
- The developed fish bile reference materials (BCR 720, BCR 721) are crucial for accurate quantification of PAH metabolites.
- These materials will enhance the reliability of field monitoring data for PAH exposure in aquatic organisms.
- Recommendations for optimal use of these reference materials are provided.