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Related Experiment Videos

False positive fecal coliform in biosolid samples assayed using A-1 medium.

Katherine H Baker1, Brady Redmond, Diane S Herson

  • 1Environmental Engineering Program, Penn State Capital College, Middletown, PA 17057, USA. khb4@psu.edu

Water Environment Research : a Research Publication of the Water Environment Federation
|August 27, 2005
PubMed
Summary

The A-1 broth method overestimates fecal coliform in biosolids compared to the standard method. This method is not recommended for regulatory compliance testing due to false positives.

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Area of Science:

  • Environmental microbiology
  • Water quality analysis
  • Biosolids treatment

Background:

  • Fecal coliforms are key indicators of fecal contamination in environmental samples.
  • Accurate enumeration of fecal coliforms is crucial for assessing the safety of treated biosolids.
  • Regulatory compliance for biosolids requires reliable pathogen reduction testing.

Purpose of the Study:

  • To compare the accuracy of two most probable number (MPN) methods for enumerating fecal coliforms in lime-treated biosolid.
  • To evaluate the suitability of the A-1 broth method for regulatory compliance testing under 40 CFR Part 503.

Main Methods:

  • Comparison of lauryl tryptose broth with Escherichia coli broth confirmation against direct A-1 broth incubation.
  • Enumeration of fecal coliforms in lime-treated biosolid samples using both MPN methods.

Related Experiment Videos

  • Analysis of positive A-1 tubes to determine the occurrence of false positives.

    • Main Results:

    • The A-1 broth incubation method yielded significantly higher fecal coliform counts.
    • A substantial percentage of positive results from the A-1 method were identified as false positives.
    • The standard method provided more reliable fecal coliform enumeration.

    Conclusions:

    • The direct A-1 broth incubation method is not recommended for fecal coliform enumeration in lime-treated biosolid.
    • The high rate of false positives makes the A-1 method unreliable for 40 CFR Part 503 Pathogen Reduction compliance testing.
    • Standard MPN methods with appropriate confirmation are preferred for accurate regulatory assessment.