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Is agNOR and DNA ploidy analysis useful for evaluating thyroid neoplasms?
Roberto Souza Camargo1, Marina Yoshie Sakamoto Maeda, Celso di Loreto
1Department of Surgery and Laboratory of Medical Investigation 26, Hospital das Clinicas, Medical School of São Paulo University, Brazil.
Analytical and Quantitative Cytology and Histology
|August 27, 2005
Summary
DNA ploidy analysis is a more valuable tool than subjective nucleolar organizer region (NOR) counting for analyzing thyroid lesions. This method showed a correlation with histologic diagnosis, unlike AgNOR counting.
Area of Science:
- Oncology
- Pathology
- Molecular Biology
Background:
- Thyroid cancer diagnosis and prognosis rely on accurate histologic evaluation.
- Novel adjunct methods are being explored to improve diagnostic accuracy and prognostic value.
Purpose of the Study:
- To correlate subjective AgNOR counting and DNA content with histologic diagnoses of thyroid cancer.
- To assess the prognostic value of DNA ploidy and AgNOR counting in thyroid carcinoma.
Main Methods:
- Eighty-one thyroid carcinoma cases and seven normal thyroids were analyzed.
- DNA quantitative measurement and DNA index determination using histograms were performed.
- Subjective counting of NORs in 200 malignant cells was conducted.
Main Results:
- DNA ploidy analysis revealed aneuploidy in a significant proportion of Hürthle cell, follicular, papillary, and undifferentiated carcinomas.
- DNA analysis showed a correlation with the histologic type of thyroid tumor (p = 0.032).
- AgNOR counting variables did not show statistical significance, and AgNOR counting lacked prognostic value.
Conclusions:
- DNA ploidy analysis is a more useful adjunct method for thyroid lesion analysis compared to subjective NOR counting.
- DNA ploidy demonstrates prognostic value, correlating with histologic diagnosis, unlike AgNOR counting.
