Related Experiment Video
Updated: Aug 16, 2026
Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
Published on: August 22, 2017
Spectrofluorimetric assay of ciprofloxacin hydrochloride in tablets
Zehra Durmus1, Esin Canel, Esma Kiliq
1Department of Chemistry, Faculty of Science, Ankara University, Ankara, Turkey. zdurmus@science.ankara.edu.tr
Objective:
To describe the spectrofluorimetric method for the determination of ciprofloxacin hydrochloride in tablets under optimum conditions.
Study Design:
Examination by spectrofluorimetry.
Results:
The wavelengths of excitation and emission were 290 and 450 nm, respectively. The fluorescence intensity was linearly related to the drug concentration over the range 2-8 microg/mL, with a limit of detection of 1.4 microg/mL and a limit of quantitation of 2.0 microg/mL. The method was highly accurate and precise, having a relative SD of < 1.0 %. The validity of the method was tested by recovery studies of the standard addition to pharmaceuticals, and the results were satisfactory. The results obtained by the application of this method and the official one were in good agreement, and statistical comparison by Student's t test and the variance ratio F test showed no significant difference between the 2 methods.
Conclusion:
The proposed method is simple and sufficiently precise for quality control purposes.
