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Updated: Aug 16, 2026

Studying Muscle Transcriptional Dynamics at Single-molecule Scales in Drosophila
Published on: September 8, 2023
Up- and downregulated genes in muscles that undergo developmentally programmed cell death in the insect Manduca sexta
Péter Löw1, Gábor Csaba Talián, Miklós Sass
1Department of Anatomy, Cell and Developmental Biology, Loránd Eötvös University, Budapest, Pázmány Péter sétány 1/C., H-1117, Hungary. peterlow@cerberus.elte.hu
Abstract:
This study was designed to investigate changes in gene expression associated with stage-specific programmed cell death (PCD) in intersegmental muscles (ISMs) of the moth, Manduca sexta. The technique of differential display reverse transcription PCR was applied to compare mRNA levels before and after the onset of PCD in ISMs. Expression of E75B transcription factor was repressed while another factor, betaFTZ-F1, stayed at a very low level. However, gene coding for a translation-initiation factor (eIF1A) was upregulated. Expression of these genes had not been previously reported to be altered in dying ISMs. An ecdysteroid agonist, RH-5849, that prevented PCD in ISMs also blocked these changes.
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