Related Experiment Video
Updated: Aug 16, 2026

Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
Expression of the cyclin-dependent kinase inhibitor p27 and its deregulation in mouse B cell lymphomas
Chen-Feng Qi1, Shao Xiang, Min Sun Shin
1Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, Twinbrook I, Room 1421, National Institutes of Health, Rockville, MD, USA.
Abstract:
CDKN1B (p27) regulates cell-cycle progression at the G1-S transition by suppressing the cyclin E/CDK2 kinase complex. In normal lymphocytes and most human B cell non-Hodgkin lymphomas (NHL), there is an inverse correlation between proliferative activity and expression of p27; however, a subset of NHL with high mitotic indices expresses p27, which is inactive due to sequestration in nuclear protein complexes or due to cytoplasmic retention. Our studies of mouse B cell NHL also identified cases with high proliferative activity and high levels of p27 at a surprisingly high frequency. Here, p27 was complexed with D-type cyclins 1 and 3 and with the COPS9 protein, JAB1. In addition, we found cytoplasmic sequestration following phosphorylation by activated AKT.
More Related Videos
Related Concept Videos
Inhibition of Cdk Activity
Inhibition of CDK Activity
Abnormal Proliferation
Negative Regulator Molecules
Positive Regulator Molecules
Positive Regulator Molecules

