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Related Experiment Videos

5 Beta-dihydroprogesterone and steroid 5 beta-reductase decrease in association with human parturition at term.

Penelope M Sheehan1, Gregory E Rice, Eric K Moses

  • 1Pregnancy Research Centre and University of Melbourne Department of Obstetrics and Gynaecology, Royal Women's Hospital, Carlton, Victoria, Australia. penny.sheehan@rwh.org.au

Molecular Human Reproduction
|August 27, 2005
PubMed
Summary
This summary is machine-generated.

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Plasma concentrations of 5beta-dihydroprogesterone (5betaDHP) decrease during labor. This progesterone metabolite

Area of Science:

  • Reproductive Endocrinology
  • Biochemistry

Background:

  • Progesterone withdrawal is controversial in human parturition.
  • Bioactive progesterone metabolites may be key to functional withdrawal.

Purpose of the Study:

  • Quantify 5beta-dihydroprogesterone (5betaDHP) plasma levels at term labor onset.
  • Measure steroid 5beta-reductase mRNA in parturition-related tissues.

Main Methods:

  • Serial blood sampling and radioimmunoassay (RIA) for 5betaDHP quantification.
  • Real-time RT-PCR to determine steroid 5beta-reductase mRNA expression.
  • HPLC for steroid separation.

Main Results:

  • 5betaDHP plasma concentration significantly decreased (two-fold) with active labor (P = 0.00001).

Related Experiment Videos

  • Steroid 5beta-reductase mRNA expression decreased in placenta (two-fold) and myometrium (10-fold) during labor.

    • Conclusions:

    • Decreased 5betaDHP levels correlate with spontaneous human labor onset.
    • Suggests a role for 5betaDHP in parturition endocrinology.
    • Further research into this pathway is warranted.