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Isolation and characterization of tributyltin chloride-resistant marine Vibrio
T Fukagawa1, S Suzuki, K Fukunaga
1Department of Food Science and Technology, Faculty of Fisheries, Hokkaido University, Japan.
FEMS Microbiology Letters
|May 15, 1992
Abstract:
Tributyltin chloride (TBTCl)-resistant marine bacteria were isolated from coastal sea water. One of these bacteria (Vibrio M-1) was highly resistant when grown in medium containing 125 microM of TBTCl. This strain was sensitive to other metals. Two polypeptides, 30 kDa and 12 kDa, increased when the strain was cultured in the medium supplemented with TBTCl. Initially TBTCl was taken up by the cell; however, the amount of TBTCl determined in the cellular fraction was low after the exponential growth phase of Vibrio M-1, suggesting the existence of a TBTCl-efflux system.