Related Experiment Video
Updated: Aug 16, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
Adsorption model for retention in normal-phase liquid chromatography with ternary mobile phases
Małgorzata Borówko1, Barbara Ościk-Mendyk
1Faculty of Chemistry, M.Curie-Skłodowska University, M.Curie-Skłodowska Sq.3. 20-031 Lublin, Poland. borowko@hektor.umcs.lublin.pl
Abstract:
The review gives a link between the theory of adsorption from multicomponent solutions and liquid chromatography. The article surveys the methods developed to describe the retention in normal-phase chromatography with ternary mobile phases with emphasis on the results of the authors. In the model used the driving force for the separation is the difference in adsorption of a solute and all solvents onto the solid surface. The general equation generates a series of simple linear relationships to predict the retention factor in ternary mobile phase for which certain parameters remain fixed. Theoretical concepts are tested by comparison with experimental data. The correlations between parameters characterizing retention in ternary, binary and pure solvents are discussed.
More Related Videos
08:54Determining Four Components in a Lipid Nanoparticle RNA Delivery System by Liquid Chromatography Combined with Evaporative Light Scattering Detector
Published on: May 30, 2025
11:44Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
Published on: January 19, 2022
Related Concept Videos
Analyte Adsorption and Distribution
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Chromatographic Methods: Classification
Chromatographic techniques are typically named by...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Chromatographic Methods: Terminology