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Updated: Aug 16, 2026

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Published on: September 21, 2017
Solution conformations of two naturally occurring RNA nucleosides: 3-methyluridine and 3-methylpseudouridine
Jean-Paul Desaulniers1, Helen M-P Chui, Christine S Chow
1Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
Abstract:
The conformations of 3-methyluridine and 3-methylpseudouridine are determined using a combination of sugar proton coupling constants from 1D NMR spectra and 1D NOE difference spectroscopy. Both C2'-endo and C3'-endo conformations are observed for 3-methyluridine (59:41, 37 degrees C, D2O) and 3-methylpseudouridine (51:49, 37 degrees C, D2O). 3-Methyluridine preferentially adopts an anti conformation in solution, whereas 3-methylpseudouridine is primarily in a syn conformation. anti/syn-Relationships are deduced by 1D NOE difference spectroscopy.
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