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Folliculogenesis

Folliculogenesis

Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...

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Related Experiment Video

Updated: Jun 24, 2026

A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System

A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System

Published on: March 15, 2011

Interactions of indole acetic acid with EGF and FSH in the culture of ovine preantral follicles.

Evelyn Rabelo Andrade¹, Marcelo Marcondes Seneda, Amauri Alcindo Alfieri

¹Faculty of Veterinary Medicine, PPGCV, State University of Ceará, Fortaleza, CE, Brazil. evelyn_andrade@yahoo.com

|August 30, 2005

Summary

This study investigated how indole acetic acid (IAA), epidermal growth factor (EGF), and follicle-stimulating hormone (FSH) affect sheep ovarian follicle growth and viability in vitro. Combining IAA with EGF or EGF with FSH best supported activated primordial follicle health during culture.

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Last Updated: Jun 24, 2026

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Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration

Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration

Published on: September 27, 2022

Area of Science:

Reproductive Biology
Developmental Biology
Ovarian Physiology

Background:

Mechanisms regulating primordial follicle exit from the non-growing pool are poorly understood.
Ovarian follicle activation is crucial for fertility and assisted reproductive technologies.

Purpose of the Study:

To evaluate the effects of indole acetic acid (IAA), epidermal growth factor (EGF), and follicle-stimulating hormone (FSH) on ovine preantral follicle growth and viability in vitro.
To determine optimal supplement combinations for maintaining follicle health during in vitro culture.

Main Methods:

Ovine ovarian cortex fragments were cultured in vitro for 2 or 6 days.
Culture media were supplemented with various combinations of IAA, EGF, and FSH.
Follicle classification (primordial vs. developing) and viability assessments were performed via histological examination.

Main Results:

All tested media supplements increased the percentage of developing follicles and decreased primordial follicles compared to controls.
Culture for 6 days reduced overall follicle viability, except in media supplemented with IAA+EGF or EGF+FSH.
IAA+EGF and EGF+FSH combinations were most effective in preserving follicle health.

Conclusions:

Indole acetic acid (IAA) and epidermal growth factor (EGF), or EGF and follicle-stimulating hormone (FSH), significantly enhance the viability of activated ovine primordial follicles during in vitro culture.
These findings provide insights into optimizing conditions for ovarian tissue culture and potential fertility preservation strategies.