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Related Experiment Videos

HIV-1 Cis Enhancing Sequence (CES) enhances CTE-dependent Gag expression.

Ornpreya Suptawiwat1, Tun-Hou Lee, Prasert Auewarakul

  • 1Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.

Virology
|August 30, 2005
PubMed
Summary

This study reveals that the HIV-1 Cis Enhancing Sequence (CES) can promote RNA export independently of the Rev protein, by interacting with the Mason-Pfizer monkey virus Constitutive Transport Element (MPMV CTE). CES functions in both Rev/RRE and CTE pathways, indicating a role in a common RNA export step.

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Area of Science:

  • Molecular Biology
  • Virology
  • Cell Biology

Background:

  • Retroviruses export intron-containing RNA using viral or cellular factors interacting with cis-elements.
  • A previously identified HIV-1 Cis Enhancing Sequence (CES) enhances Gag expression via Rev/RRE-dependent RNA export.
  • Constitutive Transport Elements (CTEs) from other viruses also mediate RNA export.

Purpose of the Study:

  • To investigate the mechanism of HIV-1 CES in RNA export.
  • To determine if CES can function independently of the Rev protein.
  • To explore the interaction of CES with other RNA export pathways, such as CTE.

Main Methods:

  • Functional analysis of CES in RNA export assays.
  • Comparison of CES activity in the presence and absence of Rev.

Related Experiment Videos

  • Investigating CES cooperation with MPMV CTE.

    • Main Results:

    • CES promotes intron-containing RNA export in a Rev-independent manner.
    • CES functions effectively by cooperating with the Mason-Pfizer monkey virus Constitutive Transport Element (MPMV CTE).
    • CES activity requires known hnRNP-A1-binding sites and other conserved sequences within CES.

    Conclusions:

    • HIV-1 CES can mediate RNA export through both Rev/RRE and CTE pathways.
    • CES likely acts at a conserved step in nuclear RNA export, downstream or upstream of Rev/RRE and CTE functions.
    • Specific sequences within CES, including hnRNP-A1 binding sites, are crucial for its function in RNA export.