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A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
Published on: March 13, 2011
[A study of the wild and captive green peafowl(Pavo muticus) by random-amplified polymorphic DNA]
Hong Chang1, Ya-Yong Ke, Ying-Juan Su
1Life School of Zhongshan University, Guangzhou 510275, China. ls49@zsu.edu.cn
Abstract:
Random-amplified polymorphic DNA (RAPD) was used to investigate the genetic diversity of the population of 14 wild green peafowl and 18 captive green peafowl (Pavo muticus). Total of 161 and 166 bands were obtained respectively, and 23 random primers were used to amplify the genomic DNA of the wild and captive green peafowls. The average relative hereditary distance of the wild and captive green peafowls is 0.0555 and 0.1355 respectively; and the Shannon diversity index is 0.4348 and 1.0163 respectively. There is a prominent difference between the two populations by T-Test of HO. All the analyses above show that the genetic diversity is very low in wild green peafowl. It tells us that the two populations come from two families by using UPGMA, which can be useful in the breeding management in the future.
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